Paper Info
Title
Generative Adversarial Networks for Morphological-Temporal Classification of Stem Cell Images
Abstract
Frequently, neural network training involving biological images suffers from a lack of data, resulting in inefficient network learning. This issue stems from limitations in terms of time, resources, and difficulty in cellular experimentation and data collection. For example, when performing experimental analysis, it may be necessary for the researcher to use most of their data for testing, as opposed to model training. Therefore, the goal of this paper is to perform dataset augmentation using generative adversarial networks (GAN) to increase the classification accuracy of deep convolutional neural networks (CNN) trained on induced pluripotent stem cell microscopy images. The main challenges are: 1. modeling complex data using GAN and 2. training neural networks on augmented datasets that contain generated data. To address these challenges, a temporally constrained, hierarchical classification scheme that exploits domain knowledge is employed for model learning. First, image patches of cell colonies from gray-scale microscopy images are generated using GAN, and then these images are added to the real dataset and used to address class imbalances at multiple stages of training. Overall, a 2% increase in both true positive rate and F1-score is observed using this method as compared to a straightforward, imbalanced classification network, with some greater improvements on a classwise basis. This work demonstrates that synergistic model design involving domain knowledge is key for biological image analysis and improves model learning in high-throughput scenarios.
Year
DOI
Venue
2022
10.3390/s22010206
SENSORS
Keywords
DocType
Volume
dataset augmentation, deep learning, developmental toxicology, generative adversarial networks, stem cell biology
Journal
22
Issue
ISSN
Citations 
1
1424-8220
0
PageRank 
References 
Authors
0.34
0
2
Name
Order
Citations
PageRank
Adam Witmer100.34
Bir Bhanu23356380.19