Title | ||
---|---|---|
A Computational approach to screen, predict and annotate human and chimpanzee PHEX intronic miRNAs, their gene targets, and regulatory interaction networks |
Abstract | ||
---|---|---|
The knowledge of what separates us genetically from our less-evolved relatives is crucial for gaining new biomedical insight about the human-chimpanzee relatedness that could influence the development of new treatments and diagnostic aids for various ailments. Especially, more than 300 diseases have been mapped to the X chromosome, which has unique and complicated characteristics than other chromosomes in the human genome. Although the genomes of humans and chimpanzees share 99% similarity, significant differences exist between the two species in their non-coding intronic regions. Therefore, this evolutionary-based genome annotation study attempted to computationally compare, contrast, and annotate the homologous miRNAs and their gene regulatory mechanisms in the intronic regions of the PHEX gene on the human X chromosome of the two species. From our results, we identified a total of 1296 human miRNAs and 46, 957 gene targets. Similarly, 30, 563 targets of homologous chimp miRNAs were predicted. miRNAs like hsa-miR-17–5p showed a maximum number of interactions while miRNAs like hsa-miR-107 with the least number of interactions in the human/chimp gene networks. A few top-ranked miRNAs such as hsa-miR-24, hsa-miR-145, hsa-miR-34a, and hsa-miR-378 were observed to be common between the two genera. The cooperativity and multiplicity of certain miRNAs were predicted to regulate the expression of diverse cancer-associated genes such as Cyclin D1, Notch1, CDK-6, E2F3, ALK4, CKDN2A, DHFR, and MAPK14. Nevertheless, further in vitro and in vivo experimental validations of these gene candidates are required before they could be used as potential diagnostic markers and drug targets. |
Year | DOI | Venue |
---|---|---|
2022 | 10.1016/j.compbiolchem.2022.107673 | Computational Biology and Chemistry |
Keywords | DocType | Volume |
PHEX gene,MiRNAs,MiRNA target prediction,Human miRNAs,Chimpanzee miRNA homologs,Gene interaction networks,MiRNA target multiplicity and cooperativity | Journal | 98 |
ISSN | Citations | PageRank |
1476-9271 | 0 | 0.34 |
References | Authors | |
0 | 10 |
Name | Order | Citations | PageRank |
---|---|---|---|
Jenifer Mallavarpu Ambrose | 1 | 0 | 0.34 |
Daniel Alex Anand | 2 | 0 | 0.34 |
Malathi Kullappan | 3 | 0 | 0.34 |
Sardar Hussain | 4 | 0 | 0.34 |
Kavin Mozhi James | 5 | 0 | 0.34 |
Radhika Nalinakumari Sreekandan | 6 | 0 | 0.34 |
Sumetha Suga Deiva Suga | 7 | 0 | 0.34 |
Devakumar Kamaraj | 8 | 0 | 0.34 |
Vishnu Priya Veeraraghavan | 9 | 0 | 0.34 |
Surapaneni Krishna Mohan | 10 | 0 | 0.34 |