Paper Info
Title
Broadband Microwave Electroporation Device for the Analysis of the Influence of Frequency, Temperature and Electrical Field Strength.
Abstract
In this paper, a broadband microwave device for cell poration is presented, that enables the analysis of the relation between frequency, electrical field strengths and temperature for a successful cell poration. Electromagnetic-thermal coupled simulations in the frequency range from 1 GHz to 10 GHz show that the device reaches electrical field strengths of 100 V/cm and temperatures lower then 40°C. Electroporation experiments with adherent C2C12 mouse myoblast cells show successful uptake of an anti-histone γ -H2A.X nanobody at a frequency of 10 GHz. This MWP device allows the fast electro-poration of adherent cells. After 15 min, the cells show uptake of γ -H2A.X-specific nanobody while most of them survived.
Year
DOI
Venue
2022
10.1109/EMBC48229.2022.9871912
Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
DocType
Volume
ISSN
Conference
2022
2694-0604
Citations 
PageRank 
References 
0
0.34
0
Authors
7
Name
Order
Citations
PageRank
Markus Paravicini100.34
Manuela Milden200.34
Laura M Pimentel Paes Frank300.34
Martin Schussler400.34
M Cristina Cardoso500.34
Rolf Jakoby61711.46
Carolin Hessinger700.34