Name
Title | ||
|---|---|---|
Fluctuation Tolerant Charge-Integration Read Scheme For Ultrafast Dna Sequencing With Nanopore Device |
Abstract | ||
|---|---|---|
A charge-integration read scheme has been developed for a solid-nanopore DNA-sequencer that determines a genome by direct and electrical measurements of transverse tunneling current in single-stranded DNA. The magnitude of the current was simulated with a first-principles molecular dynamics method. It was found that the magnitude is as small as in the sub-pico ampere range, and signals from four bases represent wide distributions with overlaps between each base. The distribution is believed to originate with translational and rotational motion of DNA in a nanopore with a frequency of over 10(5) Hz. A sequence scheme is presented to distinguish the distributed signals. The scheme makes widely distributed signals time-integrated convergent by cumulating charge at the capacitance of a nanopore device and read circuits. We estimated that an integration time of 1.4 ms is sufficient to obtain a signal difference of over 10 mV for distinguishing between each DNA base. Moreover, the time is shortened if paired bases, such as A-T and C-G in double-stranded DNA, can be measured simultaneously with two nanopores. Circuit simulations, which included the capacitance of a nanopore calculated with a device simulator, successfully distinguished between DNA bases in less than 2.0 ms. The speed is roughly six orders faster than that of a conventional DNA sequencer. It is possible to determine the human genome in one day if 100-nanopores are operated in parallel. |
Year | DOI | Venue |
|---|---|---|
2012 | 10.1587/transele.E95.C.651 | IEICE TRANSACTIONS ON ELECTRONICS |
Keywords | Field | DocType |
DNA, sequence, nanopore, time-integration read | Nanopore,Capacitance,Electrical measurements,Electronic engineering,DNA sequencing,Engineering,Time delay and integration,Electronic circuit,Ultrashort pulse,DNA sequencer | Journal |
Volume | Issue | ISSN |
E95C | 4 | 1745-1353 |
Citations | PageRank | References |
2 | 1.00 | 1 |
Authors | ||
7 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Kazuo Ono | 1 | 3 | 1.55 |
| Yoshimitsu Yanagawa | 2 | 16 | 2.95 |
| Akira Kotabe | 3 | 37 | 9.67 |
| Riichiro Takemura | 4 | 44 | 26.44 |
| Tatsuo Nakagawa | 5 | 17 | 6.77 |
| Tomio Iwasaki | 6 | 3 | 1.55 |
| Takayuki Kawahara | 7 | 318 | 103.32 |