Paper Info
Title
AYUMS: an algorithm for completely automatic quantitation based on LC-MS/MS proteome data and its application to the analysis of signal transduction.
Abstract
Comprehensive description of the behavior of cellular components in a quantitative manner is essential for systematic understanding of biological events. Recent LC-MS/MS (tandem mass spectrometry coupled with liquid chromatography) technology, in combination with the SILAC (Stable Isotope Labeling by Amino acids in Cell culture) method, has enabled us to make relative quantitation at the proteome level. The recent report by Blagoev et al. (Nat. Biotechnol., 22, 1139-1145, 2004) indicated that this method was also applicable for the time-course analysis of cellular signaling events. Relative quatitation can easily be performed by calculating the ratio of peak intensities corresponding to differentially labeled peptides in the MS spectrum. As currently available software requires some GUI applications and is time-consuming, it is not suitable for processing large-scale proteome data.To resolve this difficulty, we developed an algorithm that automatically detects the peaks in each spectrum. Using this algorithm, we developed a software tool named AYUMS that automatically identifies the peaks corresponding to differentially labeled peptides, compares these peaks, calculates each of the peak ratios in mixed samples, and integrates them into one data sheet. This software has enabled us to dramatically save time for generation of the final report.AYUMS is a useful software tool for comprehensive quantitation of the proteome data generated by LC-MS/MS analysis. This software was developed using Java and runs on Linux, Windows, and Mac OS X. Please contact ayums@ims.u-tokyo.ac.jp if you are interested in the application. The project web page is http://www.csml.org/ayums/.
Year
DOI
Venue
2007
10.1186/1471-2105-8-15
BMC Bioinformatics
Keywords
Field
DocType
spectrum,amino acid,algorithms,bioinformatics,microarrays,signal transduction,mass spectrometry,web pages,proteome,liquid chromatography,stable isotope,cell culture,tandem mass spectrometry,software requirements,computer simulation,gene expression profiling
Nat,Biology,Liquid chromatography–mass spectrometry,Tandem mass spectrometry,Stable isotope labeling by amino acids in cell culture,Proteome,Signal transduction,Mass spectrometry,Bioinformatics,DNA microarray
Journal
Volume
Issue
ISSN
8
1
1471-2105
Citations 
PageRank 
References 
17
0.47
1
Authors
8
Name
Order
Citations
PageRank
Ayumu Saito11077.23
Masao Nagasaki236826.22
Masaaki Oyama3190.83
Hiroko Kozuka-Hata4190.83
Kentaro Semba5170.47
Sumio Sugano617026.17
Tadashi Yamamoto7170.47
Satoru Miyano82406250.71