Paper Info
Title
Landscape of neutralizing assessment of monoclonal antibodies against dengue virus
Abstract
The majority of antibody binding sites (B-cell epitopes) on antigens are discontinuous. The binding between antigen and antibody is specific, but in some cases, the antibody elicited by one antigen will show cross-reactivity against other antigens. We have developed a bioinformatics-based approach for the analysis of sequence variability of neutralizing antibody binding sites and the assessment of coverage by individual neutralizing antibodies. The antigenic analysis of functional sites on the envelope (E) protein from dengue virus has been used as a case study. The description of B-cell epitopes, measurement of epitope similarity among different strains, and estimation of antibody neutralizing coverage provide insights in antibody cross-reactivity. We have defined a generalized method for the analysis of cross-reactivity of neutralizing antibodies that is also applicable to the analysis of other pathogens. This method adds to the toolset available for the characterization and the design of broadly neutralizing vaccines.
Year
DOI
Venue
2013
10.1145/2506583.2512359
BCB
Keywords
Field
DocType
neutralizing antibody,binding site,individual neutralizing antibody,neutralizing assessment,antibody cross-reactivity,generalized method,dengue virus,bioinformatics-based approach,antibody binding site,b-cell epitopes,antigenic analysis,neutralizing vaccine,monoclonal antibody
Epitope,Monoclonal antibody,Binding site,Antigen,Immunology,Computer science,Dengue virus,Cross-reactivity,Neutralizing antibody,Virology,Antibody
Conference
Citations 
PageRank 
References 
0
0.34
0
Authors
5
Name
Order
Citations
PageRank
Jing Sun100.34
G.L. Zhang220816.91
Lars R&#248/nn Olsen300.34
Ellis L. Reinherz400.34
Vladimir Brusic555163.37