Name
Title | ||
|---|---|---|
Applying combined optical tweezers and fluorescence microscopy technologies to manipulate cell adhesions for cell-to-cell interaction study. |
Abstract | ||
|---|---|---|
Cell-to-cell interactions are important for the regulation of various cell activities, such as proliferation, differentiation, and apoptosis. This paper presents an approach to studying cell-to-cell interactions at a single-cell level through manipulating cell adhesions with optical tweezers. Experiments are performed on leukemia cancer cells and stromal cells to demonstrate the feasibility of this method. After the adhesion properties of leukemia cells on stromal cells are characterized, fluorescence intensity is used as a label to study the Wnt signaling pathway of leukemia cells. The activities of the Wnt signaling pathway of K562 cells on M210B4 and HS5 cells are examined based on fluorescence analysis. The reliability of the fluorescence imaging is confirmed through comparison with traditional flow cytometry analysis. The proposed approach will offer new avenues to investigate otherwise inaccessible mechanisms in cell-to-cell interactions. |
Year | DOI | Venue |
|---|---|---|
2013 | 10.1109/TBME.2013.2255287 | IEEE Trans. Biomed. Engineering |
Keywords | Field | DocType |
m210b4 cell,cellular effects of radiation,cell activity regulation,cell-to-cell interaction,cell proliferation,traditional flow cytometry analysis,stromal cell,biomedical optical imaging,adhesion,radiation pressure,fluorescence,fluorescence imaging,k562 cell,single-cell level,cancer,optical microscopy,cell apoptosis,optical tweezers,fluorescence microscopy technology,wnt signaling pathway,leukemia cancer cell,cell adhesion manipulation,fluorescence intensity,cell-to-cell interaction study,cell differentiation,cell adhesions,hs5 cell | Wnt signaling pathway,K562 cells,Stromal cell,Fluorescence microscope,Cancer cell,Biology,Cell biology,Cell adhesion,Cell signaling,Cell–cell interaction | Journal |
Volume | Issue | ISSN |
60 | 8 | 1558-2531 |
Citations | PageRank | References |
9 | 0.79 | 2 |
Authors | ||
5 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Xue Gou | 1 | 15 | 2.07 |
| Ho Chun Han | 2 | 9 | 0.79 |
| Songyu Hu | 3 | 133 | 9.27 |
| Anskar Y H Leung | 4 | 9 | 0.79 |
| Dong Sun | 5 | 966 | 87.31 |