Paper Info
Title
The systems approach to the prespore-specific activation of sigma factor SigF in Bacillus subtilis
Abstract
The prespore-specific activation of sigma factor SigF (σF) in Bacillus subtilis has been explained mainly by two factors, i.e., the transient genetic asymmetry and the volume difference between the mother cell and the prespore. Here, we systematically surveyed the effect of these two factors on sporulation using a quantitative modeling and simulation architecture named hybrid functional Petri net with extension (HFPNe). Considering the fact that the transient genetic asymmetry and the volume difference in sporulation of B. subtilis finally bring about the concentration difference in two proteins SpoIIAB (AB) and SpoIIAA (AA) between the mother cell and the prespore, we have surveyed the effect of AB and AA concentration on the prespore-specific activation of σF occurring in the early stage of sporulation. Our results show that the prespore-specific activation of σF could be governed by the ratio of AA to AB rather than their concentrations themselves. Our model also suggests that B. subtilis could maximize the ratio of AA to AB in the prespore and minimize it in the mother cell by employing both the transient genetic asymmetry and the volume difference simultaneously. This might give a good explanation to the co-occurrence of the transient asymmetry and the volume difference during sporulation of B. subtilis. In addition, we suggest for the first time that the σF activation in the prespore might be switched off by the decrease in the ratio of AA to AB after the transient genetic asymmetry is to an end by completion of DNA translocation into the prespore.
Year
DOI
Venue
2010
10.1016/j.biosystems.2010.03.002
Biosystems
Keywords
Field
DocType
Bacillus subtilis,Sporulation,Sigma factor σF,Hybrid functional Petri net with extension (HFPNe)
Chromosomal translocation,Biology,Biophysics,DNA,Bioinformatics,Genetics,Bacillus subtilis,Sigma factor
Journal
Volume
Issue
ISSN
100
3
0303-2647
Citations 
PageRank 
References 
1
0.34
7
Authors
3
Name
Order
Citations
PageRank
Jin Hwan Do110.34
Masao Nagasaki236826.22
Satoru Miyano32406250.71