Paper Info
Title
Volume-oriented sample preparation for reactant minimization on flow-based microfluidic biochips with multi-segment mixers
Abstract
Sample preparation is one of essential processes in most biochemical reactions. In this process, raw reactants are diluted to achieve given target concentrations. So far, most of existing sample preparation techniques only consider mixing of two source solutions under the (1:1) mixing model. In this paper, we propose the first sample preparation algorithm VOSPA that not only blends several (≥ 2) solutions in a dilution operation but also allows various mixing models on flow-based microfluidic biochips with multi-segment mixers. VOSPA is a <u>v</u>olume-<u>o</u>riented <u>s</u>ample <u>p</u>reparation <u>a</u>lgorithm that enables segment-based intermediate solution reuse for better reactant minimization. Experimental results show that VOSPA can lower the reactant consumption and operation count by 72% and 59% as compared to the baseline bit-scanning method if an 8-segment mixer is used. Moreover, VOSPA outperforms an optimal algorithm, which merely allows the use of (1:1) mixing model; the reactant usage and operation count can be further reduced by 37% and 76%.
Year
Venue
Keywords
2015
DATE
biochip,sample preparation,dilution
Field
DocType
ISSN
Process engineering,Dilution,Biochip,Reuse,Computer science,Parallel computing,Flow (psychology),Microfluidics,Sample preparation,Electronic engineering,Minification,Biochemical reactions
Conference
1530-1591
Citations 
PageRank 
References 
8
0.50
13
Authors
3
Name
Order
Citations
PageRank
Chi-Mei Huang180.50
Chia-Hung Liu21699.57
Juinn-Dar Huang327027.42