Abstract | ||
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We have developed a novel technique for cutting animal cells for an automatic cloning system. Animal cells that have been chemically softened are aspirated into a narrow Teflon tube, which is then inserted into a microfluidic chip containing two orthogonal channels. One of the microchannels aspirates and fixes a cell, while the other cuts the cell with a high velocity fluid flow. The thickness of the aspiration channel is adjustable. Half the volume of the cell is aspirated into the narrow microchannel and high-speed flow is then generated to cut the cell in two. In this report, we describe the cutting and injection methods. |
Year | DOI | Venue |
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2008 | 10.1109/MEMSYS.2008.4443643 | Tucson, AZ |
Keywords | Field | DocType |
biomems,biological techniques,cellular biophysics,microchannel flow,teflon tube,animal cells cutting,aspiration channel,automatic cloning system,high-speed flow,injection methods,microchannels,microfluidic chip,orthogonal channels,chip,fluid flow | Nanotechnology,Cellular biophysics,Electronic engineering,Fluid dynamics,Microchannel,Materials science,Microfluidic chip | Conference |
ISSN | ISBN | Citations |
1084-6999 E-ISBN : 978-1-4244-1793-3 | 978-1-4244-1793-3 | 1 |
PageRank | References | Authors |
0.68 | 1 | 5 |
Name | Order | Citations | PageRank |
---|---|---|---|
Akihiko Ichikawa | 1 | 44 | 24.39 |
Seiya Takahashi | 2 | 6 | 2.65 |
Kazutsugu Matsukawa | 3 | 7 | 3.44 |
Tamio Tanikawa | 4 | 161 | 41.99 |
kohtaro ohba | 5 | 317 | 66.11 |