Title
Injection and cutting methods of animal cells using a microfluidic chip
Abstract
We have developed a novel technique for cutting animal cells for an automatic cloning system. Animal cells that have been chemically softened are aspirated into a narrow Teflon tube, which is then inserted into a microfluidic chip containing two orthogonal channels. One of the microchannels aspirates and fixes a cell, while the other cuts the cell with a high velocity fluid flow. The thickness of the aspiration channel is adjustable. Half the volume of the cell is aspirated into the narrow microchannel and high-speed flow is then generated to cut the cell in two. In this report, we describe the cutting and injection methods.
Year
DOI
Venue
2008
10.1109/MEMSYS.2008.4443643
Tucson, AZ
Keywords
Field
DocType
biomems,biological techniques,cellular biophysics,microchannel flow,teflon tube,animal cells cutting,aspiration channel,automatic cloning system,high-speed flow,injection methods,microchannels,microfluidic chip,orthogonal channels,chip,fluid flow
Nanotechnology,Cellular biophysics,Electronic engineering,Fluid dynamics,Microchannel,Materials science,Microfluidic chip
Conference
ISSN
ISBN
Citations 
1084-6999 E-ISBN : 978-1-4244-1793-3
978-1-4244-1793-3
1
PageRank 
References 
Authors
0.68
1
5
Name
Order
Citations
PageRank
Akihiko Ichikawa14424.39
Seiya Takahashi262.65
Kazutsugu Matsukawa373.44
Tamio Tanikawa416141.99
kohtaro ohba531766.11