Paper Info
Title
A microrobotic adherent cell injection system for investigating intracellular behavior of quantum dots
Abstract
This paper presents a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision XY stage. The microrobotic system demonstrates an injection speed of 25 endothelial cells per minute with a survival rate of 96% and a success rate of 82% (n=1012). The system has a high degree of performance consistency. It is immune to operator proficiency variations and from human fatigue, requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic adherent cell injection system makes the injection of thousands of adherent cells practical and will enable our testing of intracellular behavior of semiconductive quantum dots (QDs).
Year
DOI
Venue
2008
10.1109/ROBOT.2008.4543241
Pasadena, CA
Keywords
Field
DocType
cellular biophysics,medical robotics,microrobots,motion control,semiconductor quantum dots,3 degrees-of-freedom microrobot,adherent cell injection system,computer mouse clicking,computer vision microscopy,endothelial cells,intracellular behavior,motion control,semiconductive quantum dots,Adherent cell,endothelial cells,microrobotic injection,molecule screening,quantum dots
Biomedical engineering,Cellular biophysics,Human operator,Cell injection,Computer mouse,Medical robotics,Intracellular,Engineering,Microscopy,Quantum dot
Conference
Volume
Issue
ISSN
2008
1
1050-4729 E-ISBN : 978-1-4244-1647-9
ISBN
Citations 
PageRank 
978-1-4244-1647-9
3
0.49
References 
Authors
5
4
Name
Order
Citations
PageRank
Wenhui Wang19219.23
Yu Sun241869.89
Zhongfei (Mark) Zhang32451164.30
Anderson, R.4137.11