Title
High-throughput fully automated microrobotic zebrafish embryo injection
Abstract
Injection of foreign materials (e.g., DNA, RNAi, sperm, protein, and drug compounds) into individual cells has significant implications in genetics, transgenics, assisted reproduction, and drug discovery. This video presents a microrobotic system for fully automated zebraflsh embryo injection, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility. Based on computer vision and motion control, the automated microrobotic system is capable of immobilizing a large number of zebraflsh embryos into a regular pattern within seconds and injecting 15 embryos (chorion unremoved) per minute with a success rate, survival rate, and phenotypic rate all close to 100%.
Year
DOI
Venue
2008
10.1109/ROBOT.2008.4543457
Pasadena, CA
Keywords
Field
DocType
medical robotics,microrobots,motion control,robot vision,computer vision,foreign materials,fully automated microrobotic zebrafish embryo injection,human fatigue,manual operation,motion control,Microrobotic control,cell injection,computer vision,high throughput,no-tail phenotype,zebrafish embryos
Biomedical engineering,Robot vision,Cell injection,Medical robotics,Embryo,Zebrafish,Engineering,Sperm
Conference
ISSN
ISBN
Citations 
1050-4729 E-ISBN : 978-1-4244-1647-9
978-1-4244-1647-9
0
PageRank 
References 
Authors
0.34
2
3
Name
Order
Citations
PageRank
Wenhui Wang19219.23
Liu, X.Y.200.34
Yu Sun341869.89