Paper Info
Title
Characterization of Human Tear Fluid by Means of Surface-Enhanced Raman Spectroscopy.
Abstract
Tears are exceptionally rich sources of information on the health status of the eyes, as well as of whole body functionality, due to the presence of a large variety of salts and organic components whose concentration can be altered by pathologies, eye diseases and/or inflammatory processes. Surface enhanced Raman spectroscopy (SERS) provides a unique method for analyzing low concentrations of organic fluids such as tears. In this work, a home-made colloid of gold nanoparticles has been used for preparing glass substrates able to efficiently induce an SERS effect in fluid samples excited by a He-Ne laser (lambda = 633 nm). The method has been preliminary tested on Rhodamine 6G aqueous solutions at different concentrations, proving the possibility to sense substance concentrations as low as few mu M, i.e., of the order of the main tear organic components. A clear SERS response has been obtained for human tear samples, allowing an interesting insight into tear composition. In particular, aspartic acid and glutamic acid have been shown to be possible markers for two important human tear components, i.e., lactoferrin and lysozyme.
Year
DOI
Venue
2019
10.3390/s19051177
SENSORS
Keywords
Field
DocType
SERS,tear,biomedical sensors,lactoferrin,lysozyme
Lysozyme,Rhodamine 6G,Analytical chemistry,Colloidal gold,Surface-enhanced Raman spectroscopy,Colloid,Aspartic acid,Engineering,Lactoferrin,Photochemistry,Aqueous solution
Journal
Volume
Issue
ISSN
19
5.0
1424-8220
Citations 
PageRank 
References 
0
0.34
0
Authors
7
Name
Order
Citations
PageRank
Carlo Camerlingo122.80
Mikhail Lisitskiy200.68
Maria Lepore354.41
Marianna Portaccio401.35
Daniela Montorio500.34
Salvatore Del Prete680.91
Gilda Cennamo700.34