Title
An Approach for In Situ Rapid Detection of Deep-Sea Aromatic Amino Acids Using Laser-Induced Fluorescence.
Abstract
Amino acids are the material basis of almost all life activities. An improved understanding of the source, state, and cycle of amino acids is essential for determining the energy flow and material circulation of marine ecosystems. In the present study, an in situ rapid detection method of ultraviolet (UV; 266 nm) laser-induced fluorescence (LIF) technology was used to detect three natural, aromatic amino acids in the seawater. The laser-induced fluorescence peaks of aromatic amino acids tryptophan, tyrosine, and phenylalanine were located at 350 nm, 300 nm, and 280 nm, respectively. High, linear correlations between the concentrations of the aromatic amino acids and the fluorescence peak heights were observed, and the lowest detectable concentrations of tryptophan, tyrosine, and phenylalanine were 4.70 x 10(-9) mol/L, 2.76 x 10(-8) mol/L, and 6.05 x 10(-7) mol/L, respectively, which allowed us to quantify their concentrations by using laser-induced fluorescence. This paper not only provides a practical method for the detection of aromatic amino acids in seawater, but a new means to further understand the biogeochemical processes of carbon cycles in the deep sea.
Year
DOI
Venue
2020
10.3390/s20051330
SENSORS
Keywords
DocType
Volume
soluble aromatic amino acid,laser-induced fluorescence,in situ detection
Journal
20
Issue
ISSN
Citations 
5
1424-8220
0
PageRank 
References 
Authors
0.34
0
5
Name
Order
Citations
PageRank
Ranran Du100.34
Dingtian Yang200.34
Guangjia Jiang300.68
Youren Song400.34
Xiaoqing Yin500.34