Name
Title | ||
|---|---|---|
A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker |
Abstract | ||
|---|---|---|
Current assays for Clostridioides difficile in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a "turn-on " bioluminescent readout of a C. difficile-specific protease, PPEP-1. NanoLuc, a bright and stable luciferase, was "caged " with a PPEP-1-responsive peptide tail that inhibited luminescence. Upon proteolytic cleavage, the peptide was released and NanoLuc activity was restored, providing a visible readout. The bioluminescent sensor detected PPEP-1 concentrations as low as 10 nM. Sensor uncaging was achieved within minutes, and signal was captured using a digital camera. Importantly, the sensor was also functional at ambient temperature and compatible with fecal material, suggesting that it can be readily deployed in a variety of settings. |
Year | DOI | Venue |
|---|---|---|
2021 | 10.3390/s21227485 | SENSORS |
Keywords | DocType | Volume |
Clostridioides difficile, NanoLuc, point-of-care device, bioluminescent sensor, protease sensor, PPEP-1 | Journal | 21 |
Issue | ISSN | Citations |
22 | 1424-8220 | 0 |
PageRank | References | Authors |
0.34 | 0 | 6 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Kevin K Ng | 1 | 0 | 0.34 |
| Zachary E Reinert | 2 | 0 | 0.34 |
| Jeroen Corver | 3 | 0 | 0.34 |
| Danica Resurreccion | 4 | 0 | 0.34 |
| Paul J Hensbergen | 5 | 0 | 0.34 |
| Jennifer A Prescher | 6 | 0 | 0.34 |